Denaturing of single electrospun fibrinogen fibers studied by deep ultraviolet fluorescence microscopy

The mechanical properties of individual, electrospun fibrinogen fibers.

We used a combined atomic force microscopic (AFM)/fluorescence microscopic technique to study the mechanical properties of individual, electrospun fibrinogen fibers in aqueous buffer. Fibers (average diameter 208 nm) were suspended over 12 microm-wide grooves in a striated, transparent substrate. The AFM, situated above the sample, was used to laterally stretch the fibers and to measure the app...

University of Groningen Assembly dynamics of supramolecular protein - DNA complexes studied by single - molecule fluorescence microscopy

Characterizing cutaneous elastic fibers by eosin fluorescence detected by fluorescence microscopy.

BACKGROUND Though elastic fibers are as important as collagen fibers in interpretation of the histopathologic findings, it is impossible to observe them on the hematoxylin & eosin (H&E) stained specimen. OBJECTIVE Characterizing eosin fluorescence emitted by elastic fibers in H&E stained specimens. METHODS Normal skin tissue sections were stained in 4 different ways (unstained, hematoxylin ...

Characterisation of internal morphologies in electrospun fibers by X-ray tomographic microscopy.

Electrospun fabrics for use in, for example, tissue engineering, wound dressings, textiles, filters and membranes have attracted a lot of attention due to their morphological nanoscale architectures which enhance their physical properties. A thorough detailed internal morphological study has been performed on electrospun polystyrene (PS) fibers produced from dimethylformamide (DMF) solutions. I...

Deep Vascular Imaging in Wounds by Two-Photon Fluorescence Microscopy

Deep imaging within tissue (over 300 μm) at micrometer resolution has become possible with the advent of two-photon fluorescence microscopy (2PFM). The advantages of 2PFM have been used to interrogate endogenous and exogenous fluorophores in the skin. Herein, we employed the integrin (cell-adhesion proteins expressed by invading angiogenic blood vessels) targeting characteristics of a two-photo...